Nitrous Acid Reactivation of Ultraviolet-Irradiated Transforming DNA from Hemophilus influenzae
نویسنده
چکیده
Partial recovery of ultraviolet-damaged denatured or native transforming DNA from Hemophilus influenzae, has been obtained by exposing the irradiated DNA in the denatured form to nitrous acid. Some factors that affect this recovery are described. An erythromycin marker (E(20)) was not reactivated. The UV damage reactivable by nitrous acid is different from that repaired by the photoreactivating enzyme from bakers' yeast. The pretreatment with nitrous acid affords a slight protection for denatured C(25) DNA and Sm(250) DNA against ultraviolet irradiation, but this pretreatment sensitized the E(20) DNA to this irradiation.
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ورودعنوان ژورنال:
- The Journal of General Physiology
دوره 47 شماره
صفحات -
تاریخ انتشار 1963